@article {mueller_increased_2020, title = {Increased carcinoembryonic antigen expression on the surface of lung cancer cells using gold nanoparticles during radiotherapy}, journal = {Physica Medica}, volume = {76}, year = {2020}, pages = {236{\textendash}242}, abstract = {Purpose Tumor-associated antigens are a promising target of immunotherapy approaches for cancer treatments but rely on sufficient expression of the target antigen. This study investigates the expression of the carcinoembryonic antigen (CEA) on the surface of irradiated lung cancer cells in vitro using gold nanoparticles as radio-enhancer. Methods Human lung carcinoma cells A549 were irradiated and expression of CEA on the cell surface measured by flow cytometry 3~h, 24~h, and 72~h after irradiation to doses of 2~Gy, 6~Gy, 10~Gy, and 20~Gy in the presence or absence of 0.1~mg/ml or 0.5~mg/ml gold nanoparticles. CEA expression was measured as median fluorescent intensity and percentage of CEA-positive cells. Results An increase in CEA expression was observed with both increasing radiation dose and time. There was doubling in median fluorescent intensity 24~h after 20~Gy irradiation and 72~h after 6~Gy irradiation. Use of gold nanoparticles resulted in additional significant increase in CEA expression. Change in cell morphology included swelling of cells and increased internal complexity in accordance with change in CEA expression. Conclusions This study showed an increase in CEA expression on human lung carcinoma cells following irradiation. Increase in expression was observed with increasing radiation dose and in a time dependent manner up to 72~h post irradiation. The results further showed that gold nanoparticles can significantly increase CEA expression following radiotherapy.}, keywords = {Carcinoembryonic antigen, Nanoparticles, Neoantigens, Radiotherapy}, issn = {1120-1797}, doi = {10.1016/j.ejmp.2020.06.024}, url = {http://www.sciencedirect.com/science/article/pii/S1120179720301587}, author = {Mueller, Romy and Yasmin-Karim, Sayeda and DeCosmo, Kaylie and Vazquez-Pagan, Ana and Sridhar, Srinivas and Kozono, David and Hesser, Juergen and Ngwa, Wilfred} } @inbook {mueller_nanoparticle_2020, title = {Nanoparticle detection using photoacoustic imaging (PAI)}, booktitle = {Nanoparticle Enhanced Radiation Therapy}, series = {2053-2563}, year = {2020}, note = {Type: Book Chapter}, pages = {14{\textendash}1 to 14{\textendash}8}, publisher = {IOP Publishing}, organization = {IOP Publishing}, abstract = {Chapter 14 provides a review of photo-acoustic imaging methods for macroscopic mapping of nanoparticles in patients or animals.}, isbn = {978-0-7503-2396-3}, doi = {10.1088/978-0-7503-2396-3ch14}, url = {http://dx.doi.org/10.1088/978-0-7503-2396-3ch14}, author = {Mueller, Romy and Ngwa, Wilfred and Hesser, Juergen} } @inbook {hesser_x-ray_2020, title = {X-ray based nanoparticle imaging}, booktitle = {Nanoparticle Enhanced Radiation Therapy}, series = {2053-2563}, year = {2020}, note = {Type: Book Chapter}, pages = {12{\textendash}1 to 12{\textendash}18}, publisher = {IOP Publishing}, organization = {IOP Publishing}, abstract = {Chapter 12 provides a review of x-ray imaging methods for macroscopic mapping of nanoparticles in patients or animals.}, isbn = {978-0-7503-2396-3}, doi = {10.1088/978-0-7503-2396-3ch12}, url = {http://dx.doi.org/10.1088/978-0-7503-2396-3ch12}, author = {Hesser, Juergen and Brivio, Davide} } @conference {wang2019gpu, title = {GPU Acceleration of Wave Based Transmission Tomography}, booktitle = {2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI 2019)}, year = {2019}, pages = {1753{\textendash}1756}, publisher = {IEEE}, organization = {IEEE}, author = {Wang, Hongjian and Huynh, Thai Hoa and Gemmeke, Hartmut and Hopp, Torsten and Hesser, Juergen} } @article {Loew2019, title = {Multiple retrieval case-based reasoning for incomplete datasets}, journal = {Journal of Biomedical Informatics}, volume = {In Press, Accepted Manuscript}, year = {2019}, doi = {10.1016/j.jbi.2019.103127}, url = {https://doi.org/10.1016/j.jbi.2019.103127}, author = {Loew, Nikolas and Hesser, Juergen and Blessing, Manuel} } @article {blessing2018automated, title = {Automated ultrafast kilovoltage{\textendash}megavoltage cone-beam CT for image guided radiotherapy of lung cancer: System description and real-time results}, journal = {Zeitschrift f{\"u}r Medizinische Physik}, volume = {28}, number = {2}, year = {2018}, pages = {110{\textendash}120}, publisher = {Elsevier}, author = {Blessing, Manuel and Arns, Anna and Wertz, Hansjoerg and Stsepankou, Dzmitry and Boda-Heggemann, Judit and Hesser, Juergen and Wenz, Frederik and Lohr, Frank} } @article {verma2018method, title = {Method for assessing the impact of residual roughness after corneal ablation in polychromatic vision}, journal = {Investigative Ophthalmology \& Visual Science}, volume = {59}, number = {9}, year = {2018}, pages = {5806{\textendash}5806}, publisher = {The Association for Research in Vision and Ophthalmology}, author = {Verma, Shwetabh and Hesser, Juergen and Mosquera, Samuel Arba} } @article {Verma2017, title = {Optimum Laser Beam Characteristics for Achieving Smoother Ablations in Laser Vision Correction}, journal = {Investigative Opthalmology {\&} Visual Science}, volume = {58}, year = {2017}, month = {apr}, pages = {2021}, abstract = {

Purpose Controversial opinions exist regarding optimum laser beam characteristics for achieving smoother ablations in laser-based vision correction. The purpose of the study was to outline a rigorous simulation model for simulating shot-by-shot ablation process. The impact of laser beam characteristics like super Gaussian order, truncation radius, spot geometry, spot overlap, and lattice geometry were tested on ablation smoothness. Methods Given the super Gaussian order, the theoretical beam profile was determined following Lambert-Beer model. The intensity beam profile originating from an excimer laser was measured with a beam profiler camera. For both, the measured and theoretical beam profiles, two spot geometries (round and square spots) were considered, and two types of lattices (reticular and triangular) were simulated with varying spot overlaps and ablated material (cornea or polymethylmethacrylate [PMMA]). The roughness in ablation was determined by the root-mean-square per square root of layer depth. Results Truncating the beam profile increases the roughness in ablation, Gaussian profiles theoretically result in smoother ablations, round spot geometries produce lower roughness in ablation compared to square geometry, triangular lattices theoretically produce lower roughness in ablation compared to the reticular lattice, theoretically modeled beam profiles show lower roughness in ablation compared to the measured beam profile, and the simulated roughness in ablation on PMMA tends to be lower than on human cornea. For given input parameters, proper optimum parameters for minimizing the roughness have been found. Conclusions Theoretically, the proposed model can be used for achieving smoothness with laser systems used for ablation processes at relatively low cost. This model may improve the quality of results and could be directly applied for improving postoperative surface quality.

}, issn = {1552-5783}, doi = {10.1167/iovs.16-21025}, url = {http://www.ncbi.nlm.nih.gov/pubmed/28384723 http://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.16-21025}, author = {Verma, Shwetabh and Hesser, Juergen and Arba-Mosquera, Samuel} } @article {Arns2017, title = {Phantom-based evaluation of dose exposure of ultrafast combined kV-MV-CBCT towards clinical implementation for IGRT of lung cancer}, journal = {PLOS ONE}, volume = {12}, year = {2017}, month = {nov}, pages = {e0187710}, abstract = {

PURPOSE Combined ultrafast 90{\textdegree}+90{\textdegree} kV-MV-CBCT within single breath-hold of 15s has high clinical potential for accelerating imaging for lung cancer patients treated with deep inspiration breath-hold (DIBH). For clinical feasibility of kV-MV-CBCT, dose exposure has to be small compared to prescribed dose. In this study, kV-MV dose output is evaluated and compared to clinically-established kV-CBCT. METHODS Accurate dose calibration was performed for kV and MV energy; beam quality was determined. For direct comparison of MV and kV dose output, relative biological effectiveness (RBE) was considered. CT dose index (CTDI) was determined and measurements in various representative locations of an inhomogeneous thorax phantom were performed to simulate the patient situation. RESULTS A measured dose of 20.5mGE (Gray-equivalent) in the target region was comparable to kV-CBCT (31.2mGy for widely-used, and 9.1mGy for latest available preset), whereas kV-MV spared healthy tissue and reduced dose to 6.6mGE (30{\%}) due to asymmetric dose distribution. The measured weighted CTDI of 12mGE for kV-MV lay in between both clinical presets. CONCLUSIONS Dosimetric properties were in agreement with established imaging techniques, whereas exposure to healthy tissue was reduced. By reducing the imaging time to a single breath-hold of 15s, ultrafast combined kV-MV CBCT shortens patient time at the treatment couch and thus improves patient comfort. It is therefore usable for imaging of hypofractionated lung DIBH patients.

}, issn = {1932-6203}, doi = {10.1371/journal.pone.0187710}, url = {http://www.ncbi.nlm.nih.gov/pubmed/29125857 http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5681289 http://dx.plos.org/10.1371/journal.pone.0187710}, author = {Arns, Anna and Blessing, Manuel and Fleckenstein, Jens and Stsepankou, Dzmitry and Boda-Heggemann, Judit and Hesser, Juergen and Lohr, Frank and Wenz, Frederik and Wertz, Hansjoerg}, editor = {Zhang, Qinghui} } @inbook {Prabhune2016, title = {Prov2ONE: An Algorithm for Automatically Constructing ProvONE Provenance Graphs}, booktitle = {Provenance and Annotation of Data and Processes}, year = {2016}, pages = {204{\textendash}208}, publisher = {Springer, Cham}, organization = {Springer, Cham}, doi = {10.1007/978-3-319-40593-3_22}, url = {http://link.springer.com/10.1007/978-3-319-40593-3{\_}22}, author = {Prabhune, Ajinkya and Zweig, Aaron and Stotzka, Rainer and Gertz, Michael and Hesser, Juergen} } @conference {Prabhune2015, title = {An Optimized Generic Client Service API for Managing Large Datasets within a Data Repository}, booktitle = {2015 IEEE First International Conference on Big Data Computing Service and Applications}, year = {2015}, publisher = {IEEE}, organization = {IEEE}, keywords = {abstract interface, application program interfaces, biological specimen datasets, biomedical optical imaging, Cache storage, client-server systems, Command Line Tool, Communities, Computer architecture, Data transfer, data warehouses, digital data repository system, download process, GCS API, Generic Client Service (GCS) API, high-resolution images, high-resolution microscopy, ingest process, KIT Data Manager, large dataset management, Large Datasets, Large Scale Data Repository, Localization Microscopy (LM), medical computing, meta data, Metadata, Metadata Management, Microscopy, Nanoscopy, nanoscopy scientific research community, optical microscopy, optimized generic client service API, scientific information systems, scientific research data, Workflow}, isbn = {978-1-4799-8128-1}, doi = {10.1109/BigDataService.2015.25}, url = {http://ieeexplore.ieee.org/articleDetails.jsp?arnumber=7184863}, author = {Prabhune, Ajinkya and Stotzka, Rainer and Jejkal, Thomas and Hartmann, Volker and Bach, Margund and Schmitt, Eberhard and Hausmann, Michael and Hesser, Juergen} } @article {jmuders03, title = {Projection-Wise Filter Optimization for Limited-Angle Cone-Beam CT Using the Approximate Inverse}, journal = {IEEE Transactions on Nuclear Science}, volume = {62}, year = {2015}, month = {02/2015}, pages = {148,163}, chapter = {1}, abstract = {

In this paper, we present a novel and efficient approach for the optimization of filters for limited-angle cone beam computed tomography (CBCT). Our method is based on the theory of approximate inverse (AI) and uses a simultaneous iterative reconstruction technique (SIRT) to estimate a view-dependent reconstruction kernel. From this kernel we then derive a set of 2-D filters that can be applied in a filtered backprojection (FBP) algorithm. By construction the resulting filters are independent of the measured data, so that they can be precomputed for a given geometric setup and be reused with different projection datasets. Our approach is the first application of the AI for 3-D limited-angle CBCT supported by iterative reconstruction, such that in comparison to existing methods, it does not rely on additional reference measurements or on the existence of an analytical inversion formula. However, our method reaches results better than standard FBP methods. Additionally, we provide a general scheme that allows the transfer of our method to other system geometries and gives us the ability to extend it with more complex filters. We will conduct several experiments with simulated and real data where we examine the image quality of our method in comparison to standard FBP and SIRT. The results will show that our angle-optimized FBP has a higher contrast-to-artifact ratio than FBP. In addition to this, we analyze the image quality perpendicular to the in-focus plane by the use of the artifact spread function and show that our technique can be employed to reduce the amount of ghosting artifacts.

}, issn = {0018-9499}, author = {Muders, Jens and Hesser, Juergen} }